document.write('<a href="http://www.cshblogs.org/cshprotocols/2010/03/08/total-internal-reflection-fluorescence-microscopy-tirfm/" title="Total Internal Reflection Fluorescence Microscopy (TIRFM)"  target=_blank style="font-size:120%;font-weight:bold;color:#036;">Total Internal Reflection Fluorescence Microscopy (TIRFM)</a><br><a href="http://www.cshblogs.org/cshprotocols/2010/03/08/total-internal-reflection-fluorescence-microscopy-tirfm/"  target=_blank style="line-height:20px;font-size:90%;color:#666666;text-decoration:none;">Mon, 08 Mar 2010 20:01:43 +0000</a><br>The recent explosion in the availability and variety of fluorescent proteins, new organic dyes and quantum dots has been a driving force in the growing use of Total Internal Reflection Fluorescence Microscopy (TIRFM).  TIRFM only illuminates molecules that are within a thin volume near the coverslip surface of a specimen and not those deeper [...]');